Pichia pastoris HCP ELISA kit DEIABL491 from Creative Diagnostics

Product Specs
ItemPichia pastoris HCP ELISA kit
CompanyCreative Diagnostics
Price Supplier Page View Company Product Page
Catalog NumberDEIABL491
Quantity96T
ReactivityPichia pastoris
Sample TypePichia pastoris host cells
ApplicationsELISA
Detection TargetPichia pastoris HCP
Components1. Clear 96-Well Strip Plate
Clear plastic strip-well microtiter plate coated with rabbit anti-Pichia HCP IgG. Can be used as individual strips.
2. Pichia Protein Standard (1024 ng/ml, 600 µl)
Concentrated Pichia proteins sufficient for generating a standard curve from 512 ng/ml to 8 ng/ml.
3. 5x Dilution Buffer (15 ml)
1x Dilution Buffer is used for dilution of Reporting Antibody and Streptavidin-HRP conjugate. 1x Dilution Buffer is used to dilute samples if necessary. The 10 ml of 5x concentrate should be diluted to 50 ml with 40 ml of milliQ water to achieve 1x Dilution Buffer.
4. 10x PBS-T (30 ml)
1x PBS-T is used for wash steps. The 25 ml of 10x concentrate should be diluted to 250 ml with 225 ml of milliQ water to achieve 1x PBS-T.
5. Reporting Antibody (90µl/tube)
A biotin labeled rabbit polyclonal antibody specific for Pichia cell proteins. Immediately prior to the assay, dilute 75 µl into 15 ml of 1x Dilution Buffer.
6. Streptavidin-HRP Conjugate (4 µg/ml, 420 µl/tube)
A Streptavidin–Horse Radish Peroxidase conjugate in a stabilizing solution. Immediately prior to the assay, dilute 375 µl into 15 ml of 1x Dilution Buffer to give a 0.1 µg/ml working stock.
7. TMB Substrate (15 ml)
Use directly without dilution.
8. Stop Solution (15 ml)
A 1M solution of sulfuric acid. CAUSTIC. Use directly without dilution. The solution should not come in contact with skin or eyes. Take appropriate precautions when handling this reagent.
9. Plate Sealer
SensitivityThe lower limit of detection (LOD) is defined as that concentration corresponding to a signal two standard deviations above the mean of the zero standard. LOD is ~0.1 ng/mL.
The lower limit of quantitation (LOQ) is defined as the lowest concentration where concentration coefficients of variation (CVs) are less than 20%. LOQ is ~0.5 ng/mL

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Creative Diagnostics

45-1 Ramsey Road

Shirley, N 11967

United States

Phone: 1-631-624-4882 (USA)
44-161-818-6441 (Europe)

Email: info@creative-diagnostics.com

Company Profile

Website: https://www.creative-diagnostics.com/

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Pichia pastoris HCP ELISA kit from Creative Diagnostics

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Creative Diagnostics

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Bench Tips for Optimizing ELISA: It All Comes Out in the Wash

Pichia pastoris HCP ELISA kit from Creative Diagnostics

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